DNA dependent-RNA polymerases fromPhysarum polycephalum
نویسندگان
چکیده
منابع مشابه
Purification and properties of two RNA polymerases from Physarum polycephalum.
Two RNA polymerases have been purified from the slime mold Physarum polycephalum, one sensitive and one resistant to alpha-amanitin. Both enzymes are more active with denatured DNA than native DNA as a template and prefer Mn(++) rather than Mg(++) as a divalent cation. The alpha-amanitin-sensitive enzyme shows maximum activity at 0.15 M KCl, whereas the resistant enzyme is most active at very l...
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The properties of DNA-dependent RNA polymerases have been studied since the 1960s, but considerable interest in probing RNA polymerase structure/function relationships, the roles of different classes of RNA polymerases in cellular processes, and the feasibility of using RNA polymerases as drug targets still exists. Historically, RNA polymerase activity has been measured by the incorporation int...
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DNA-dependent RNA polymerases of immature and castrated rat uteri were studied after estradiol administration. The enzymes were solubilized from either whole uterus homogenate or nuclei and their activities were measured on an exogenous DNA template. oc-Amanitin was used to distinguish Lu-amanitin-resistant from cu-amanitin-senitive forms of the enzyme. The number of oc-amanitin-sensitive RNA p...
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The DNA-dependent RNA polymerases of members of the three orders of methanogens were purified and their enzymatic properties described. The enzymes consist of 7-8 polypeptides. Although these differed in molecular mass, the four heaviest components could be allied to components of the enzyme of Methanobacterium thermoautotrophicum, W by cross-reaction with antibodies directed against the denatu...
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ژورنال
عنوان ژورنال: FEBS Letters
سال: 1973
ISSN: 0014-5793
DOI: 10.1016/0014-5793(73)80572-1